Structural Biology: new methods Text Karl-Franzens-Universitaet Homepage of Karl Franzens Universität Graz Homepage of Institut für Chemie, Universität Graz
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We have developed a method to diffuse noble gases into protein crystals at elevated pressures (up to 100 bar) and to freeze these crystals without desorption of the bound gas. This technique is suitable for the preparation of isomorphous derivatives to be used for the solution of the crystallographic phase problem. A second less trivial application concern phase-contrast experiments to visualize regions of lipids or detergents in crystals of membrane proteins.
 
Technical drawing of the current version of our Xenon-cell
 
This research was supported by a joint project of our group with a number of other groups in Germany, Italy, France and the UK funded by the European Commission. The EXMAD (Extension of Capabilities for MAD Experiments at Synchrotron Infrastructures) project has the objective of devloping tools for macromolecular crystallography.

Hyperlink to EXMAD-homepage
 
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Nov.15, 2004         Christoph.Kratky@uni-graz.at                                                            HOME              UNI GRAZ